T4573
Sigma
Tris Acetate-EDTA buffer
working solution, Biotechnology Performance Certified
| Synonym: | TAE buffer |
| MDL number: | MFCD00236357 |
Description
| Application | TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis.1 Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity.2 Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution. |
| General description | Solution contains 40 mM Tris acetate and 1 mM EDTA at an approximate pH of 8.3. Made with WFI water and 0.2 μm filtered. |
| Preparation Note | Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258). |
Properties
| grade | Biotechnology Performance Certified |
| sterility | sterile; 0.2 μm filtered |
| total impurities | DNase, RNase, Protease, tested |
| bioburden, tested | |
| endotoxin, tested | |
| ≤5 ppm heavy metals (as Pb) | |
| suitability | suitable for gel electrophoresis (after dilution to working concentration) |
Safety
| WGK Germany | 2 |
References
| Cited Reference | 1. Sambrook, J., et al. Molecular Cloning: A Laboratory Manual Cold Spring Harbor, , (1989) 23, 6.7 |
| 2. Loening, U.E., The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem. J. 102, 251-257, (1967) | |
| reference | Ogden, R.C., and Adams, D.A., Electrophoresis in agarose and acrylamide gels. Meth. Enzymol. 152, 61-87, (1987) |
